Karl F. Ehrlich, Ph.D. and Marie-Claude Cantin Ph.D.
Copyright © 1995
[I have reformatted this document to be more readable and consistentEd.]
A healthy aquarium allows us to bring a fragment of the wondrous and beautiful aquatic world into our homes or work place. Contemplation of our aquatic microcosm brings us tranquility as greater harmony and understanding of nature's splendid intricacies.
An aquarium may contain fishes and/or invertebrates; plants may or may not also be present. The animals are what we invest in and whose beauty and behaviour we appreciate; yet we confine them in an environment in which they feed and their wastes can accumulate. The ability to maintain the animals in good health is a function of the efficiency and health of the aquarium's wastewater treatment facility: the biological filter and the aquarist's management of the system.
Water purification is accomplished by teams of beneficial bacteria working together in an appropriate environment. If the proper microorganisms are not present or if the correct physico-chemical conditions are not maintained, the biological filters will not function adequately. When this happens our pets will be stressed, suffer and loose their beauty; they can even die.
Stress is caused by many factors including poor water quality (principally in terms of ammonia, nitrite, pH, hardness or oxygen levels), or food (virtually always too much and sometimes poor quality), incompatible mixtures of aquarium residents (either intra- and/or interspecific), and/or lack of appropriate physical environment (e.g., too bright, too much disturbance, lack of or wrong physical substrate, no shelter) etc. Stress is cumulative; each stress combines with another to increase the overall stress upon the individual. This applies to not only to fishes but also to us. Just as we may quite often be exposed to diseases without always being sick, increased stress often results in increased susceptibility to disease; the same applies to our aquarium residents. A properly designed and operating biological filter in a well managed aquarium will maintain good water quality and eliminate the major stressful conditions for aquarium animals.
Beneficial microorganisms within an aquarium convert ammonia, produced by the animals and from the breakdown of excess food and wastes, into nitrite and then into nitrate. The cycling of an aquarium is the process of establishing an equilibrium between the synthesis of ammonia and its conversion into nitrate by beneficial microorganisms. City water is sterile and contains none of the needed bacteria. Cycle Biological Supplement and Cycle Sludge Removers have been designed to assure the presence of a balanced community of water purifying microorganisms.
Under-gravel filters can provide a very large surface for biological filtration. This is particularly the case when power heads are used to increase water movement through the gravel. Once the nitrifying bacteria, in Cycle Biological Supplement have been added, the filter will remove ammonia and nitrite. Under-gravel filters, however, also filter the water physically, collecting solids such as fish wastes and excess food. Efficient nitrification requires movement of oxygenated water around the nitrifying microorganisms.
Excessive accumulation of solids, within the under-gravel filter has several detrimental effects upon nitrification. First of all, as water movement through the gravel, and oxygen concentration within the gravel decrease, the useful surface area within the filter and the efficiency of nitrification or the removal of ammonia and nitrite also decrease. Secondly, waste accumulations in the increasingly anoxic gravel result in synthesis of ammonia, soluble phosphorous and biochemical oxygen demand (BOD) from the sludge and production of nitrite from partial denitrification (see Nitrification). High BOD levels can inhibit nitrification. The toxicity syndrome is due to the combined reduction in nitrification coupled with increased production of ammonia and nitrite. The transition from beneficial to detrimental conditions within an unmanaged under-gravel filter can be quite rapid. This explains the too often observed phenomenon of the toxicity syndrome in established aquaria, when animals suddenly die after several months of normal operation.
Excessive accumulations of sludge, whether in an under-gravel filter or elsewhere in an aquarium, initiate the processes leading to the toxicity syndrome. Cycle Freshwater Sludge Removers contain communities of beneficial microorganisms and enzymes selected for their ability to remove sludge and to prevent the problems associated with the toxicity syndrome.
Aquarium Water Chemistry
Aquarium health management requires some understanding of water chemistry. Information on selected important parameters is provided below.
Phosphorous - There is much talk by aquariophiles about the evils
of phosphorous. Certain realities, however, should be kept in mind.
Nitrogen - There are five forms of nitrogen: gas, organic, ammonia, nitrite and nitrate. The relationship between the different forms of nitrogen in known as the nitrogen cycle.
Air is 80% nitrogen, and this gas is not toxic at normal concentrations in water. The only problem results from supersaturation associated with changes in operating pressure, such as when water is released from deep reservoirs or air is sucked into a high pressure pump. Aquarium water does not usually have this problem. Nitrogen supersaturation causes the bends in divers as well as in fishes. Symptoms of nitrogen supersaturation include bubbles in blood vessels and bulging eyes.
Organic Nitrogen is found in fish food, their wastes, sludge as well as the animals and plants. All life consists of approximately 16% nitrogen. Proteins can be soluble or insoluble. Protein molecules are generally too large to pass through cell membranes and be metabolized. Certain, but not all bacteria are able to produce enzymes, which solubilize the proteins to smaller molecules. These can then be biodegraded by the microbes to produce cell biomass, carbon dioxide and water. Cycle Sludge Removers not only provide this essential part of the water purifying community but also ones which solubilize fats and carbohydrates.
Fish excrete ammonia from their gills. Ammonia is produced by the animals as a function of nitrogenous wastes. This process is a part of the Nitrogen Cycle in an aquarium. There are two forms of ammonia, unionized (NH3) and ionized (NH4+). Only the unionized form is toxic. An equilibrium exists in water between the quantity of NH3 and NH4+; the percentage of the toxic form increases with the temperature and pH (Fig. 4). Thus at 25° C., in fresh water, less than 0.1% of the total ammonia is in the toxic form, but at pH 8.5 NH3 accounts for more than 10% - an increase of over 100 times. Ammonia is less toxic at lower pH levels. Most test kits measure the sum of both forms of ammonia. The only way to know how much ammonia is in the toxic form is by determining the pH and temperature and then calculating the percentage in toxic form. The long-term safe level of ammonia varies with different species, but the value of 0.02 ppm of NH3 is often used. If a test kit gives a reading of 1 ppm for total ammonia at 25° C. in fresh water this means that at pH 7.0 the toxic form is 0.6% of the total or 1 ppm x 0.006 = 0.006 ppm NH3
and there is no problem, but at pH 8.5 the toxic form is 15% of the total or 1 ppm x 0.15 = 0.05 ppm NH3 and there is a problem.
Another factor to consider is the units that the test kit measures. Ammonia contains nitrogen (N) and hydrogen (H). Some kits may report total ammonia, as described above, but other kits refer to the quantity of nitrogen in the ammonia molecule. In this case the test kits units are presented as total ammonia nitrogen (TAN), which is the sum of NH3-N+NH4-N. Results reported in the two forms are not the same. It is necessary to know the percentage of nitrogen in the ammonia molecule to compare the two units. The atomic weight of nitrogen is 14 and that of hydrogen is 1; thus the molecular weight of NH3 is 14+3 = 17. Nitrogen is 14/17 or 82% of the weight of the ammonia molecule. The difference is much more important for nitrite and nitrate (see below). Thus 1 ppm of NH3 is the same thing as 0.82 ppm of NH3-N. It is essential to know what units are being measured with each test kit. The bacteria in Cycle Biological Supplement remove ammonia by converting it to nitrite and then to nitrate (see Nitrification below). Other strains in Cycle Biological Supplement and Cycle Sludge Removers also consume ammonia as a nitrogen source for their growth.
Nitrite is produced from ammonia as well as from nitrate. Excessive nitrite (NO2-) is toxic to aquatic organisms particularly in fresh water. The toxicity of nitrite is greatly reduced in saltwater. The long-term safe level of NO2- varies with different species; 0.1 & 0.2 ppm are considered safe limits in soft and hard water respectively. As for ammonia, test kits give results in terms of nitrite or nitrite-nitrogen (NO2-N). The difference between the two units is greater than for ammonia. The atomic weight of oxygen is 16; thus the molecular weight of NO2 is 14+32=46. Nitrogen is 14/46 or 30% of the weight of the nitrite molecule. Thus a test kit reading of 1 ppm of NO2 is the same thing as 0.3 ppm of NO2-N. It is essential to know what units are being measured with each test kit.
Nitrate is the final product of nitrification, and it tends to accumulate in aquaria without living plants. Nitrate of even above 100 ppm have been shown to be nontoxic to many species. Nitrate accumulation in aquaria can be controlled by regular water changes and use of live plants. Bacteria in Cycle are also able to convert nitrate to nitrogen gas under special conditions.